Custom Gene Synthesis
With world's top-level expert team, most advanced equipment, and sophisticated gene design and optimization software, General Biosystems can synthesize genes of any length and complexity to suit your needs. We can synthesize and clone any DNA sequences (even complex, GC-rich, repeated, or long genes) into prokaryotic, eukaryotic or viral vectors. Genes up to 3,000 bp are normally synthesized in 1-2 weeks!
Features & Benefits
  • Free codon optimization upon request.
  • Accept genes over 40-kb in length.
  • Flexible cloning choices: standard cloning, custom cloning and GB Seamless Cloning.
  • Free subcloning into one of our standard vectors.
  • As low as $49 for subcloning into any vector of our choice.
  • As fast as 5 Business Days, on average 2 weeks turn around time.
  • 100% sequence-guaranteed synthetic genes of the highest quality.

  • Guarantee: General Biosystems will guarantee appropriate genes (see Terms & Conditions) to ship from General Biosystems within the above turnaround times once an order has been approved. Appropriate genes that fail to meet the business day turnaround time posted will be credited with half the gene cost on your General Biosystems account (could be used for your further orders).

    Terms & Conditions : Our turnaround guarantee is not valid for orders requiring custom cloning, genes with high GC/AT, stretches of homopolymers, low copy vectors, extensive repeats or genes longer than 3kb. Turnaround times can be affected if a gene is toxic to E.coli at propagation. Orders placed before 5:00pm EST (on USA business days) will be considered ordered on the same day.

    Service Procedure
    Service Options
    Sequence Length Delivery time Price
    <500bp 5~8 Business days $99/Gene
    500bp~1499bp 8~10 Business days $0.19/bp
    1500bp~2999bp 12~14 Business days $0.19/bp
    3000bp~4999bp 16~22 Business days $0.30/bp
    >5000bp Inquire Inquire

    Standard Deliverables
    - 4-5 ug Lyophilized DNA in tubes or plates
    - Plasmid map (Electronic)
    - Sequence chromatograms covering your gene (electronic)
    - Certificate of Analysis (COA)
    Special notice: We promise not to release or use customer's gene sequence or other related information in any forms. We will destroy all primers, plasmids and strains after the products have been delivered for one month.

    For batch orders, please order our High-throughput Gene Synthesis Service starting from as low as $0.16/bp (Min order size: 10 genes).
    Vector Information
    -Our standard vector is pUC57/pBlueScript II SK(+). Synthetic genes will be cloned into standard vector for free.
    -Cloning into our in stock commercial vectors is also free (NEW!). Please click here to view the vector maps.
    -Any other vectors must be provided by the customer along with full-length sequence and/or map. Now we offer free, secure storage of your custom plasmid vectors or commercial vectors service, and only $29 cloning into any archived vectors bundled with future gene synthesis orders.
    -Worrying about the customs clearance problems? Please contact us to inquire about our distributors.
    BioCat GmbH: Exclusive Distributor for Germany, Austria and Switzerland.

    Some Peer-reviewed Citations:
    Elevated serum autoantibodies against co-inhibitory PD-1 facilitate T cell proliferation and correlate with disease activity in new-onset systemic lupus erythematosus patients
    https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-017-1258-4
    Hui Shi, Junna Ye, Jialin Teng, Yufeng Yin, Qiongyi Hu, Xinyao Wu, Honglei Liu, Xiaobing Cheng, Yutong Su, Mengru Liu, Juanfang Gu, Ting Lu, HaoJie Chen, Hui Zheng, Yue Sun Chengde Yang-Arthritis Research & Therapy ... All subjects signed written informed consent. Production of recombinant human PD-1 extracellulardomain. The extracellular domain of the human PD-1 gene (NP_005009.2) Leu24-Gln167 was synthesized by General Biosystems Inc. (Morrisville, NC, USA)...

    Isomaltulose production by yeast surface display of sucrose isomerase from Pantoea dispersa on Yarrowia lipolytica
    http://www.sciencedirect.com/science/article/pii/S1756464617301093
    L Li, H Wang, H Cheng, Z Deng - Journal of Functional Foods
    ... NG_047456] with hp4d promoter at its upstream, yielding the plasmid pHyg-Pir1. The SIase gene from P. dispersa (PdSIase, GenBank accession no. AY223549) was optimized according to the codon bias of Y. lipolytica, synthesized chemically (General Biosystems Inc. ...
    Ordering Information
    Or download and fill out the order form below and email to order@generalbiosystems.com. Our sales representative will contact you within 24 hours.