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Parameters of common dyes used for primer modification


    Dye

    Maximum absorption wavelength (nm)

    Fluorescence emission maximum wavelength (nm)

    Molar extinction coefficient

    Colour

    FAM

    495

    520

    83,000

    Green

    TET

    521

    536

    73,000

    Orange

    JOE

    520

    548

    71,000

    Pink

    HEX

    520

    548

    71,000

    Pink

    TAMRA

    544

    576

    90,000

    Rose

    Cy3

    550

    570

    150,000

    Red

    ROX

    576

    601

    82,000

    Red

    TexasRed

    583

    603

    107,000

    Red

    Cy5

    650

    670

    250,000

    Violet