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Electrophoresis Basic Parameters

    Separation range (bp)

    Recommended % agarose

    Separation range (bp)

    Recommended % 
    Polyacrylamide Gel

    1,000~30,000

    0.5%

    100~1,000

    3.5%

    800~12,000

    0.7%

    80~500

    5.0%

    500~10,000

    1.0%

    60~400

    8.0%

    400~7,000

    1.2%

    40~200

    12.0%

    200~4,000

    1.5%

    5~100

    20.0%

    50~2,000

    2.0%

     

     


    PAGE concentration

    Size of fragments in nucleotides, bp

    Bromophenol blue

    Xylene Cyanol FF

    3.5%

    100

    460

    5.0%

    65

    260

    8.0%

    45

    160

    12.0%

    20

    70

    15.0%

    15

    60

    20.0%

    12

    45